HIV P24 Rapid Test Kit - Fast & Reliable Detection
PRINCIPLE
The HIV 1/2 Human Immunodeficiency Virus Rapid Test Device (Whole Blood/Serum/Plasma) is a qualitative, membrane based immunoassay for the detection of antibodies to HIV 1/2 in whole blood, serum or plasma. The membrane is pre‐coated with recombinant HIV antigens. During testing, the whole blood, serum or plasma specimen reacts with HIV antigen coated particles in the test strip. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with recombinant HIV antigen on the membrane in the test line region. If the specimen contains antibodies to HIV 1 and/or HIV 2, a colored line will appear in the test line region indicating a positive result. If the specimen does not contain HIV 1 and/or HIV 2 antibodies, a colored line will not appear in the test line region indicating a negative result. To serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
Product Detail
Brand: QL
Specimens: : Serum/Plasma
Reading time：10 minutes.
Pack：25 T
STORAGE: 2‐30°C
KIT COMPONENTS(Device)
Test devices
Disposable specimen droppers
Buffer
Package insert
Brand: QL
Specimens: : Whole Blood/Serum/Plasma
Reading time：Strip：15 minutes. Device：10 minutes
Pack：25 T
STORAGE: 2‐30°C
KIT COMPONENTS（Device）
Test devices
Disposable specimen droppers
Buffer
Package insert
KIT COMPONENTS（Strip）
Test strips
Disposable specimen droppers
Buffer
Package insert
Test cards
DIRECTIONS FOR USE
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HIV 1/2 Rapid Test Strip(Whole Blood/Serum/Plasma)
Allow the test strip, specimen, buffer, and/or controls to equilibrate to room temperature (15‐30°C) prior to testing.
1. Remove the test strip from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
2. Peel off the tape from the test card, and stick the test strip in the middle of test card with arrows pointing down on the test card.
For Serum, Plasma or Venipuncture Whole Blood specimen: Hold the dropper vertically and transfer 2 drops of serum, plasma or venipuncture whole blood (approximately 80 L) to the
“Specimen Pad” of the test strip, then add 1 drop of buffer (approximately 40L) and start the timer.
For Fingerstick Whole Blood specimen:
To use a capillary tube: Fill the capillary tube and transfer approximately 80L of fingerstick whole blood specimen to the “Specimen Pad” of the test strip, then add 1 drop of buffer (approximately 40L) and start the timer.
To use hanging drop: Allow 2 hanging drops of fingerstick whole blood specimen to fall into the center of the “Specimen Pad” on the test strip, then add 1 drop of buffer (approximately
40L) and start the timer.
3. Wait for the colored line(s) to appear. The result should be read at 15 minutes. Do not interpret results after 20 minutes.
HIV 1/2 Rapid Test Strip（Serum/Plasma）
HIV 1/2 Rapid Test Device (Whole Blood/Serum/Plasma)
Allow the test device, specimen, buffer, and/or controls to equilibrate to room temperature
(15‐30°C) prior to testing.
1. Remove the test device from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
2. Place the test device on a clean and level surface. Hold the dropper vertically and transfer 2 drops of serum, plasma (approximately 80L) to the specimen well (S) of the sample pad then add 1 drop of buffer (approximately 40L) and start thetimer.
3. Wait for the colored line(s) to appear. The result should be read at 10 minutes. Do not interpret results after 20 minutes.
HIV 1/2 Rapid Test Device（Serum/Plasma）
Allow the test device, specimen, buffer, and/or controls to equilibrate to room temperature (15‐30°C) prior to testing.
1. Remove the test device from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
2. Place the test device on a clean and level surface.
For Serum, Plasma specimen: Hold the dropper vertically and transfer 2 drops of serum, plasma (approximately 80L) to the specimen well (S) of the test device, then add 1 drop of buffer (approximately 40L) and start the timer.
3. Wait for the colored line(s) to appear. The result should be read at 10 minutes. Do not interpret results after 20 minutes.
INTERPRETATION OF RESULTS
INVALIDRESULT:
Control line (C) falls to appear. Insufficient buffer volume or incorrect procedural techniques are the most likely reasons for control line failure.
Review the procedure and repeat the procedure with a new test device. If the problem persists, discontinue using the test kit immediately and contact your local distributor.
*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of HIV antibodies present in the specimen. Therefore, any shade of colored in the test region (T) should be considered positive.
The core of the HIV P24 Rapid Test Kit lies in its innovative technology, designed to detect the p24 antigen, a protein found in the HIV virus, as well as antibodies to HIV 1 and HIV 2. This dual detection capability enhances the test's sensitivity, allowing for the identification of HIV infections at an earlier stage compared to antibody-only tests. The test can be administered using whole blood, serum, or plasma samples, making it a versatile option for various testing environments, from clinical settings to privacy of one's home. Understanding the principle behind the HIV P24 Rapid Test Kit is essential for appreciating its significance in HIV detection. When a sample is applied to the test device, it reacts with the synthetic antigens coated on the membrane, revealing the presence of either HIV p24 antigen or antibodies to HIV 1/2 if present in the sample. This process unfolds within minutes, providing a qualitative result that is easy to interpret. It's a breakthrough in the field, offering hope and empowering individuals with the knowledge they need to seek timely medical intervention.